Hydroaminase (HAM)
Apejuwe kukuru:
Nipa Hydroaminase
Awọn oriṣi 2 ti ọja henensiamu HAM (Nọmba bi ES-HAM-101 ~ ES-HAM-102) ni idagbasoke nipasẹ SyncoZymes.HAM le mu amoniation ti enoic acid tabi awọn itọsẹ rẹ lati gbe awọn amino acids chiral jade.HAM le ṣee lo lati ṣepọ awọn amino acids chiral (tabi amines chiral) lati awọn enoic acids (tabi alkenes).Oluranlọwọ Amonia, gẹgẹbi omi amonia tabi iyọ ammonium, jẹ pataki.
Hydroaminase katalitiki lenu iru
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Imeeli:lchen@syncozymes.com
| Awọn enzymu | koodu ọja | Sipesifikesonu |
| Enzymu Powder | ES-HAM-101~ ES-HAM-102 | ṣeto ti 2 Nuclosidephosporyalse, 50 mg kọọkan 2 awọn ohun kan * 50mg / ohun kan, tabi awọn miiran opoiye |
Eto ifasẹyin atẹle le ṣee lo fun itọkasi:
1-10 mg / milimita: sobusitireti
10 miligiramu / milimita: enzymu
10% (v/v): Organic cosolvent (1)
10% (v/v): Amonia
Ṣatunṣe pH si 9.0 ṣaaju fifi enzymu kun, ati enzymu yẹ ki o ṣafikun nikẹhin.Ṣafikun awọn aati ni gbigbọn otutu igbagbogbo (fun apẹẹrẹ, 30oC, 150rpm) ninu fun awọn wakati 24 ~ 48.Centrifuge adalu kọọkan lati ṣaju amuaradagba, wọn le ṣee lo supernatant fun TLC, HPLC tabi itupalẹ GC.
Akiyesi (1): Eroja Organic (gẹgẹbi DMSO, methanol, acetonitrile, IPA, ati bẹbẹ lọ) jẹ iyan, ti a lo fun awọn sobusitireti insoluble.
