Nitrile reductase (NRED)
Apejuwe kukuru:
Nipa Nitrile reductase
Iru 3 wa ti ọja enzymu NRED (Nọmba bi ES-NRED101 ~ ES-NRED103) ti dagbasoke nipasẹ SyncoZymes.NRED le ṣe itọsi idinku ti ẹgbẹ nitrile si amine.Ninu ilana ti catalysis, NADPH ni a nilo bi olupona hydrogen.
Nitrile reductasecatalytic lenu iru
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Imeeli:lchen@syncozymes.com
| Awọn enzymu | koodu ọja | Sipesifikesonu |
| Sipesifikesonu | ES- NRED -101~ ES- NRED -103 | ṣeto ti 3 Nitrile Reductase, 50 miligiramu kọọkan 3 awọn ohun kan * 50mg / ohun kan, tabi awọn miiran opoiye |
Eto ifasẹyin atẹle le ṣee lo fun itọkasi:
1-10 mg / milimita sobusitireti
10 mg / milimita enzymu
10% (v/v) Organic cosolvent (1)
100 mM Fọsifate ifipamọ (pH7.0)
0.2 mg/ml NADP+
10 mg / milimita glukosi
2 mg/ml Glucose dehydrogenase (GDH)
Ṣafikun awọn aati ni gbigbọn otutu igbagbogbo (fun apẹẹrẹ, 30oC, 150rpm) ninu fun awọn wakati 24 ~ 48.Centrifuge adalu kọọkan lati ṣaju amuaradagba, wọn le ṣee lo supernatant fun TLC, HPLC tabi itupalẹ GC.
Akiyesi (1): Eroja Organic (gẹgẹbi DMSO, methanol, acetonitrile, IPA, ati bẹbẹ lọ) jẹ iyan, ti a lo fun awọn sobusitireti insoluble.
