Transaminase (ATA)
Awọn enzymu: Ṣe awọn oludasọna ti ibi-ara macromolecular, pupọ julọ awọn enzymu jẹ awọn ọlọjẹ.
Transaminases: Kilasi ti awọn ensaemusi ti o mu ki amino gbigbe laarin amino acids ati keto acids.Awọn transaminases jẹ awọn enzymu ti ibi pataki ni iṣelọpọ asymmetric ati ipinnu ije ti awọn amines chiral.
Aminotransferase le pin si awọn kilasi mẹrin ni ibamu si ọna ati ilana: Ⅰ, Ⅱ, Ⅲ ati Ⅳ.ω-aminotransferases jẹ ti awọn transaminases kilasi II, ti o wọpọ ni a lo ni igbaradi ti amines chiral ati awọn amino acids ti ko ni ẹda, gẹgẹbi β-amino acids.
ω-aminotransferases: Ni ọpọlọpọ awọn ọran, ω-transaminase tọka si kilasi kan ti awọn enzymu, ti awọn aati gbigbe amonia catalytic laisi α-amino acid bi sobusitireti tabi ọja.
Ilana katalitiki:
| Awọn enzymu | koodu ọja | koodu ọja |
| Enzymu Powder | ES-ATA-101~ ES-ATA-165 | ṣeto ti 65 ω-Transaminases, 50 mg kọọkan 65 awọn ohun kan * 50mg / ohun kan, tabi awọn miiran opoiye |
| Apo iboju (SynKit) | ES-ATA-6500 | ṣeto ti 65 ω-Transaminases, 1 miligiramu kọọkan 65 awọn ohun kan * 1mg / ohun kan |
★ Ga sobusitireti pato.
★ Alagbara chiral selectivity.
★ Ga iyipada ṣiṣe.
★ Kere nipasẹ-ọja.
★ Ìwọnba lenu ipo.
★ Ore ayika.
Ṣiṣayẹwo Enzyme yẹ ki o ṣe fun awọn sobusitireti kan pato nitori iyasọtọ sobusitireti, ati gba enzymu kan ti o ṣe itọsi sobusitireti ibi-afẹde pẹlu ipa ayase to dara julọ.
➢ Maṣe kan si awọn ipo to gaju gẹgẹbi: iwọn otutu giga, giga / pH kekere ati ohun elo Organic pẹlu ifọkansi giga.
Ni deede, eto ifaseyin yẹ ki o pẹlu sobusitireti, ojutu ifipamọ, oluranlowo amino (bii amino acids ati 1-phenyl ethylamine) tabi olugba (bii keto acids), coenzyme (PLP), cosolvent (gẹgẹbi DMSO).
➢ ATA yẹ ki o ṣafikun nikẹhin sinu eto ifaseyin, lẹhin pH ati iwọn otutu ti ni titunse si ipo ifura.
➢ Gbogbo iru ATA ni ọpọlọpọ awọn ipo ifaseyin to dara julọ, nitorinaa ọkọọkan wọn yẹ ki o ṣe iwadi ni ẹyọkan.
Apeere 1 (ikojọpọ ti Sitagliptin, iṣelọpọ asymmetric)(1):
Apẹẹrẹ 2 (Mexiletine, Apapo ipinnu kainetic pẹlu iṣelọpọ asymmetric)(2):
1 Savile CK, Janey JM, Mundorff EC, ati al.Imọ, 2010, 329 (16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Organic awọn lẹta, 2009,11 (21): 4810-4812.
