CALB aibikita
CALB jẹ aibikita nipasẹ adsorption ti ara lori resini hydrophobic giga ti o jẹ macroporous, styrene/methacrylate polima.CALB aibikita jẹ o dara fun awọn ohun elo ni awọn ohun elo Organic ati awọn ọna ṣiṣe ti ko ni epo, ati pe o le tunlo ati tun lo fun awọn akoko pupọ ni awọn ipo to dara.
ọja Code: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★ Iṣẹ ṣiṣe ti o ga julọ, yiyan chiral ti o ga julọ ati iduroṣinṣin to ga julọ.
★ Iṣe ti o dara julọ ni awọn ipele ti kii ṣe olomi.
★ Ni irọrun yọkuro kuro ninu eto ifaseyin, yiyara fopin si awọn aati, ki o yago fun iyoku amuaradagba ninu ọja.
★ Le tunlo ati tun lo lati ge iye owo naa.
| Iṣẹ-ṣiṣe | ≥10000PLU/g |
| pH ibiti fun lenu | 5-9 |
| Iwọn iwọn otutu fun esi | 10-60 ℃ |
| Ifarahan | CALB-IMMO100-A: Ina ofeefee to brown ri to CALB-IMMO100-B: Funfun to ina brown ri to |
| Iwọn patiku | 300-500μm |
| Pipadanu lori gbigbe ni 105 ℃ | 0.5% -3.0% |
| Resini fun immobilization | Macroporous, styrene/methacrylate polima |
| epo ifa | Omi, Organic epo, bbl, tabi laisi epo.Fun iṣesi ni diẹ ninu awọn olomi Organic, 3% omi ni a le ṣafikun lati mu ipa iṣesi dara si |
| Iwọn patiku | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Itumọ apakan: Ẹyọ 1 ni ibamu si iṣelọpọ ti 1μmol fun iṣẹju kan propyl laurate lati lauric acid ati 1-propanol ni 60 ℃.CALB-IMMP100-A ti o wa loke ati CALB-IMMO100-B ni ibamu si awọn aruji ti a ko gbe pẹlu awọn titobi patiku oriṣiriṣi.
1. riakito iru
Enzymu aibikita jẹ iwulo fun riakito ipele ipele kettle mejeeji ati riakito ṣiṣan lilọsiwaju ibusun ti o wa titi.O yẹ ki o ṣe akiyesi lati yago fun fifun pa nitori agbara ita nigba ifunni tabi kikun.
2. Reaction pH, otutu ati epo
Enzymu aibikita yẹ ki o ṣafikun nikẹhin, lẹhin awọn ohun elo miiran ti a ṣafikun ati tituka, ati atunṣe pH.
Ti agbara ti sobusitireti tabi dida ọja yoo ja si iyipada ti pH lakoko ifura, ifipamọ to yẹ yẹ ki o ṣafikun si eto ifaseyin, tabi pH yẹ ki o ṣe abojuto ati ṣatunṣe lakoko iṣesi.
Laarin iwọn ifarada iwọn otutu ti CALB (ni isalẹ 60 ℃), oṣuwọn iyipada pọ si pẹlu ilosoke ti iwọn otutu.Ni lilo ilowo, iwọn otutu ifaseyin yẹ ki o yan ni ibamu si iduroṣinṣin ti sobusitireti tabi ọja.
Ni gbogbogbo, iṣesi ester hydrolysis jẹ o dara ni eto alakoso olomi, lakoko ti iṣesi iṣelọpọ ester dara ni eto alakoso Organic.Ohun elo Organic le jẹ ethanol, tetrahydrofuran, n-hexane, n-heptane ati toluene, tabi epo idapọmọra ti o dara.Fun iṣesi ni diẹ ninu awọn olomi Organic, 3% omi ni a le ṣafikun lati mu ipa iṣesi dara si.
3. Atunlo ati igbesi aye iṣẹ ti CALB
Labẹ ipo ifaseyin ti o yẹ, CALB le gba pada ati tun lo, ati awọn akoko ohun elo kan pato yatọ pẹlu awọn iṣẹ akanṣe oriṣiriṣi.
Ti CALB ti o gba pada ko ba tun lo nigbagbogbo ati pe o nilo lati wa ni ipamọ lẹhin imularada, o nilo lati fo ati ki o gbẹ ati ki o di edidi ni 2-8 ℃.
Lẹhin ọpọlọpọ awọn iyipo ti ilotunlo, ti ṣiṣe iṣe iṣe ba dinku diẹ, CALB le ṣafikun ni deede ati tẹsiwaju lati lo.Ti o ba ti ṣiṣe ifaseyin dinku ni pataki, o nilo lati paarọ rẹ.
Apẹẹrẹ 1 (Aminolysis)(1):
Apẹẹrẹ 2 (Aminolysis)(2):
Apeere 3(Akopọ polyester ti nsii oruka)(3):
Apeere 4(Transesterification, regioselective of hydroxyl group)(4):
Apẹẹrẹ 5 (Iyipada Iyipada, kainetik ipinnu ti racemic alcohols)(5):
Apẹẹrẹ 6(Esterification, ipinnu kinetic ti carboxylic acid)(6):
Apẹẹrẹ 7 (Esterolysis, ipinnu kinetic)(7):
Apẹẹrẹ 8 (Hydrolysis ti amides)(8):
Apẹẹrẹ 9 (Acylation of amines)(9):
Apeere 10(Idahun afikun Aza-Michael)(10):
1. Chen S, Liu F, Zhang K, ati Tal.Tetrahedron Lett, 2016, 57: 5312-5314.
2. Olah M, Boros Z, anszky GH, e Tal.Tetrahedron, 2016, 72: 7249-7255.
3. Nakaoki1 T, Mei Y, Miller LM, etal.Ind Biotechnol, 2005, 1 (2): 126-134.
4. Pawar SV, Yadav G DJ Ind.Ọdun 2015, 31: 335-342.
5. Kamble MP, Shinde SD, Yadav G DJ Mol.Catal.B: Iṣiro, 2016, 132: 61-66.
6. Shinde SD, Yadav G D. ilana Biochem, 2015, 50: 230-236.
7. Souza TC, Fonseca TS, Costa JA, e Tal.J. Mol.Catal.B: Iṣiro, 2016, 130: 58-69.
8. Gavilan AT, Castillo E, Lopez-Mungu'AJ Mol.Catal.B: Iṣiro, 2006, 41: 136-140.
9. Joubioux FL, Henda YB, Bridiau N, e Tal.J. Mol.Catal.B: Iṣiro, 2013, 85-86: 193-199.
10. Dhake KP, Tambade PJ, Singhal RS, e Tal.Tetrahedron Lett, 2010, 51: 4455-4458.
