Esterase&Lipase (PLE&CALB)

Apejuwe kukuru:

Nipa Esterase&Lipase

ES-PLEs: Kilasi ti awọn hydrolases ti o jẹ ki dida ati fifọ awọn iwe adehun ester.Wọn le kopa ninu ọpọlọpọ awọn iru awọn aati biokemika, gẹgẹbi esterification, transesterification ati hydrolysis.Wọn ti wa ni lilo pupọ ni ọpọlọpọ awọn aaye bii ṣiṣe ounjẹ, ile-iṣẹ kemikali, biomedicine, oogun chiral ati itọju ayika.

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Imeeli:lchen@syncozymes.com

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Nipa Esterase&Lipase:

Awọn iru 26 ti awọn ọja enzymu PLE wa (Nọmba bi ES-PLE-101～ES-PLE-126) ti a dagbasoke nipasẹ SyncoZymes.ES-PLE le ṣee lo fun hydrolysis ti aliphatic ati awọn agbo ogun ester, tabi ipinnu regioselective ati stereoselective lati ṣajọpọ awọn acids chiral ati awọn itọsẹ wọn.

Irisi ifasilẹ katalitiki:

Awọn anfani:

★ Ga sobusitireti pato.
★ Alagbara chiral selectivity.
★ Ga iyipada.
★ Kere nipasẹ-ọja.
★ Ìwọnba lenu ipo.
★ Ore ayika.

Awọn ilana fun lilo:

Ni deede, eto ifaseyin yẹ ki o pẹlu sobusitireti, ojutu ifipamọ ati ES-PLE.Esterification ti diẹ ninu awọn ES-PLE ni a ṣe ni ipele Organic.
➢ Gbogbo iru awọn ES-PLE ti o baamu si ọpọlọpọ awọn ipo ifaseyin to dara julọ yẹ ki o ṣe iwadi ni ẹyọkan.

Awọn apẹẹrẹ elo:

Apẹẹrẹ 1 (Biosynthesis ti Pregabalin Intermediate)⁽¹⁾:

Apeere 2⁽²⁾:

Apeere 3⁽³⁾:

Apeere 4⁽⁴⁾:

Ibi ipamọ:

Jeki 2 ọdun ni isalẹ -20 ℃.

Ifarabalẹ:

Maṣe kan si awọn ipo to gaju gẹgẹbi: iwọn otutu giga, giga/kekere pH.

Awọn itọkasi:

1. Xu FX, Chen SY, Xu G, e Tal.App.Biotechnol Bioproc E, 1988, 54 (4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD etal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e Tal.Synlett, ọdun 1994, ọdun 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917

Ti tẹlẹ: Epoxide hydrolase (EH)

Itele: Ṣe agbekalẹ dehydrogenase (FDH)

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