Cytochrome P450 monooxygenase (CYP)

Apejuwe kukuru:

Nipa CYP

ES-CYPs: Kilasi ti awọn ensaemusi redox pẹlu mercaptan-heme gẹgẹbi ile-iṣẹ ti nṣiṣe lọwọ lati ṣe itọsi gbigbe atẹgun.Wọn jẹ oxygenases ebute ati nilo heme lati mu atẹgun ṣiṣẹ.

Cytochrome P450 monooxygenase ṣe itọsi ifoyina, epoxideation ati hydroxylation ti alkyl, hydroxylation ati oxidation ti amonia, ifoyina ti sulfur, dealkylation (atẹgun, sulfur, amonia), dehydrogenation oxidative, deamination ati dehalogenation.
Awọn oriṣi 8 ti awọn ọja cytochrome P450 monooxygenase (Nọmba bi ES-CYP-101～ES-CYP-108) ni idagbasoke nipasẹ SyncoZymes.

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Imeeli:lchen@syncozymes.com

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Alaye ọja

ọja Tags

Nipa CYP:

Iru ifaseyin katalitiki:

Alaye ọja:

Awọn enzymu	Apo iboju (SynKit)	Sipesifikesonu
Enzymu Powder	ES-CYP-101~ ES-CYP-108	ṣeto ti 8 Cytochrome P450 Monooxygenases, 50 miligiramu kọọkan 8 awọn ohun kan * 50mg / ohun kan, tabi opoiye miiran
Apo iboju (SynKit)	ES-CYP-800	ṣeto ti 8 Cytochrome P450 Monooxygenases, 1mg kọọkan 8 awọn ohun kan * 1mg / ohun kan

Awọn anfani:

★ Broad sobusitireti julọ.Oniranran.
★ Ga iyipada.
★ Kere nipasẹ-ọja.
★ Ìwọnba lenu ipo.
★ Ore ayika.

Awọn ilana fun lilo:

Ni deede, eto ifaseyin yẹ ki o pẹlu sobusitireti, ojutu ifipamọ (PH ti o dara julọ ti henensiamu), coenzyme (NAD (H) tabi NADP (H)), eto isọdọtun coenzyme (fun apẹẹrẹ glucose ati glukosi dehydrogenase) ati ES-CYP.coenzyme ati eto isọdọtun coenzyme le rọpo nipasẹ hydrogen peroxide ni eto ifaseyin apa kan.
➢ Gbogbo iru awọn ES-CYPs ti o baamu si ọpọlọpọ awọn ipo ifaseyin to dara julọ yẹ ki o ṣe iwadi ni ẹyọkan.
➢ Idojukọ giga tabi ọja pẹlu le ṣe idiwọ iṣẹ ES-CYP.Bibẹẹkọ, idinamọ le ni itunu nipasẹ afikun ipele ti sobusitireti.

Awọn apẹẹrẹ elo:

Apeere 1⁽¹⁾:

Apeere 2⁽²⁾:

Apeere 3⁽³⁾:

Apeere 4⁽⁴⁾:

Ibi ipamọ:

Jeki 2 ọdun ni isalẹ -20 ℃.

Ifarabalẹ:

Maṣe kan si pẹlu awọn ipo to gaju gẹgẹbi: iwọn otutu giga, giga / pH kekere ati idalẹnu Organic fojusi giga.

Awọn itọkasi:

1. Zaretzki J, Matlock M, ati Swamidass S JJ Chem.Inf.Awoṣe, 2013, 53, 3373-3383.
2. Gannett P M., Kabulski J, Perez F A., ati Tal.J. Am.Chem.Soc., 2006, 128 (26), 8374-8375.
3. Cryle M J., Matovic N J., ati De Voss J J. Org.Lett., 2003, 5 (18), 3341-3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e Tal.Biochim.Biophys.Ìṣirò, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK ati Tal.Acta Crystallogr.Ọdun 2009, Ọdun 65, Ọdun 372.