Nucloside phosphoryalse (NP)

Apejuwe kukuru:

Nipa nucleoside phosphoryalse

Awọn iru mẹta ti awọn ọja enzymu NP wa (Nọmba bi ES-NP-101 ~ ES-NP-103) ti a dagbasoke nipasẹ SyncoZymes.ES-NP-101 jẹ purine nucleoside phosphorylase, ES-NP-102 ati ES-NP-103 jẹ pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase le decompose awọn nucleosides sinu awọn ipilẹ ati pentose fosifeti.Nucleoside phosphorylase ni a le pin si purine nucleoside phosphorylase ati pyrimidine nucleoside phosphorylase ni ibamu si ayanfẹ ti awọn ipilẹ nucleoside.Purine nucleoside phosphorylase le ṣe metabolize adenosine si adenine, inosine si hypoxanthine, guanosine si guanine, ati gbejade fosifeti ribose ni akoko kanna.Pyrimidine nucleoside phosphorylase le ṣe metabolize uridine si uracil ati gbejade fosifeti ribose.

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Alaye ọja

ọja Tags

Nucloside phosphoryalse lenu iru:

Alaye ọja:

Awọn enzymu	koodu ọja	Sipesifikesonu
Enzymu Powder	ES-NP-101~ ES-NP-103	ṣeto ti 3 Nuclosidephosporyalse, 50 mg kọọkan 3 awọn ohun kan * 50mg / ohun kan, tabi awọn miiran opoiye

Ilana awọn aati iboju ti Nucloside phosphoryalse:

Eto ifasẹyin atẹle le ṣee lo fun itọkasi:
1-10 mg / milimita sobusitireti
10 mg / milimita enzymu
100 mM Fọsifate ifipamọ (pH7.0)
Ṣafikun awọn aati ni gbigbọn otutu igbagbogbo (fun apẹẹrẹ, 30oC, 150rpm) ninu fun awọn wakati 12 ~ 24.Centrifuge kọọkan adalu lati precipitate amuaradagba, wọn awọn supernatant le ṣee lo fun TLC, HPLC tabi GC onínọmbà.