Leucine Dehydrogenase (LeuDH)
Apejuwe kukuru:
Nipa Leucine dehydrogenase
Iru 1 wa ti ọja enzymu LeuDH (Nọmba bi ES-LeuDH-101) ti dagbasoke nipasẹ SyncoZymes.LeuDH le ṣe itọsi amoniation idinku ti 4-methylbutanoic acid (iyọ) si L-leucine tabi ifoyina ti leucine si 4-methylbutanoic acid.ES-LeuDH tun le mu ifasilẹ idinku ti α-keto acids pẹlu eto ti o jọra lati mura awọn itọsẹ amino acid ti o baamu.Ninu ilana ti catalysis, NADH jẹ pataki bi gbigbe hydrogen.
Leucine dehydrogenasecatalytic lenu iru
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Imeeli:lchen@syncozymes.com
| Awọn enzymu | koodu ọja | Sipesifikesonu |
| Enzymu Powder | ES-LeuDH-101 | ṣeto ti 1 Monoamine Oxidase, 50 miligiramu kọọkan awọn ohun kan 1 * 50mg / ohun kan, tabi opoiye miiran |
Eto ifasẹyin atẹle le ṣee lo fun itọkasi:
1-10 mg / milimita: sobusitireti
10 miligiramu / milimita: enzymu
10% (v/v): Organic cosolvent (1)
100 milimita: Ifipamọ Phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 miligiramu/ml: Fọọmu dehydrogenase (FDH)
Ṣafikun awọn aati ni gbigbọn otutu igbagbogbo (fun apẹẹrẹ, 30oC, 150rpm) ninu fun awọn wakati 24 ~ 48.Centrifuge kọọkan adalu lati precipitate amuaradagba, wọn awọn supernatant le ṣee lo fun TLC, HPLC tabi GC onínọmbà.
Akiyesi (1): Eroja Organic (gẹgẹbi DMSO, methanol, acetonitrile, IPA, ati bẹbẹ lọ) jẹ iyan, ti a lo fun awọn sobusitireti insoluble.
