Ene reductase (ERED)
Awọn ES-ERED ṣe itọsi ọpọlọpọ awọn iru sobusitireti nitori iwoye nla ti awọn sobusitireti.Ni gbogbogbo, C = C ti α, β-unsaturated agbo pẹlu awọn ẹgbẹ elekitironi (pẹlu ketone aldehyde, awọn ẹgbẹ nitro, acids carboxylic, esters, anhydride, lactones, imines, ati bẹbẹ lọ) ni irọrun dinku nipasẹ ES-EREDs, ṣugbọn awọn unactivated ė ìde ko.
Awọn iru 46 wa ti awọn ọja enzymu ERED (Nọmba bi ES-ERED-101~ES-ERED-146) ti a dagbasoke nipasẹ SyncoZymes.
Ilana katalitiki:
Awọn enzymu | koodu ọja | Sipesifikesonu |
Enzymu Powder | ES-ERED-101 ~ ES-ERED-146 | ṣeto ti 46 Ene Reductases, 50 mg kọọkan 46 awọn ohun kan * 50mg / ohun kan, tabi awọn miiran opoiye |
Apo iboju (SynKit) | ES-ERED-4600 | ṣeto ti 46 Ene Reductases, 50 mg kọọkan 46 awọn ohun kan * 50mg / ohun kan, tabi awọn miiran opoiye |
★ Ga sobusitireti pato.
★ Alagbara chiral selectivity.
★ Ga iyipada.
★ Kere nipasẹ-ọja.
★ Ìwọnba lenu ipo.
★ Ore ayika.
★ ga ailewu.
Ni deede, eto ifaseyin yẹ ki o pẹlu sobusitireti, ojutu ifipamọ (pH ifaseyin ti o dara julọ), coenzymes (NAD (H) tabi NADP (H)), eto isọdọtun coenzyme (fun apẹẹrẹ glucose ati glukosi dehydrogenase) ati ES-ERED.
➢ Gbogbo awọn ES-ERED ni a le ṣe idanwo ni ọkọọkan ninu eto ifasẹyin loke tabi pẹlu Apo iboju ERED (SynKit ERED).
➢ Gbogbo iru ES-ERED ti o ni ibamu si ọpọlọpọ awọn ipo ifaseyin to dara julọ yẹ ki o ṣe iwadi ni ẹyọkan.
➢ Idojukọ giga tabi ọja pẹlu le ṣe idiwọ iṣẹ ES-ERED.Bibẹẹkọ, idinamọ le ni itunu nipasẹ afikun ipele ti sobusitireti.
Apẹẹrẹ 1 (α, β-unsaturated aldehydes tabi ketones)(1):
Apẹẹrẹ 2 (α, β-unsaturated carboxylic acids ati awọn itọsẹ wọn)(2):
Jeki 2 ọdun ni isalẹ -20 ℃.
Maṣe kan si pẹlu awọn ipo to gaju gẹgẹbi: iwọn otutu giga, giga / pH kekere ati idalẹnu Organic fojusi giga.
1. Lucídio C, Fardelone J, Augusto R, ati Tal.J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, ati Tal..Org.Lett, 2007, 9 (26): 5409-5411.