Amidase (AMD)
Awọn enzymu:Ni o wa macromolecular ti ibi catalysts, julọ ensaemusi ni o wa awọn ọlọjẹ
Amidase:Ṣe itọsi hydrolysis ti ọpọlọpọ endogenous ati ajeji aliphatic ati awọn amides aromatic nipa gbigbe ẹgbẹ acyl kan si omi pẹlu iṣelọpọ awọn acids ọfẹ ati amonia.Awọn acids hydroxamic ati awọn acids Organic miiran jẹ lilo pupọ bi awọn oogun nitori wọn jẹ awọn ipin ti awọn ifosiwewe idagba, awọn oogun aporo ati awọn inhibitors tumo.Awọn amidases le pin si iru R ati iru acylases S ni ibamu si stereoselectivity ayase.
Ni afikun si sisọ hydrolysis ti amides, amidase tun le ṣe itọsi awọn aati gbigbe acyl ni iwaju awọn sobusitireti bi hydroxylamine.
Amidase pẹlu awọn orisun oriṣiriṣi ni pato sobusitireti oriṣiriṣi, diẹ ninu wọn le ṣe hydrolyze awọn amides aromatic nikan, diẹ ninu wọn le ṣe hydrolyze aliphatic amides nikan, ati diẹ ninu awọn hydrolyze α-tabi ω-amino amides.Pupọ ninu awọn amines ni iṣẹ katalitiki to dara nikan fun acyclic tabi awọn amides aromatic ti o rọrun, ṣugbọn fun awọn aromatics eka, awọn amides heterocyclic, paapaa awọn amides pẹlu awọn aropo ortho, ni gbogbogbo ni iṣẹ-ṣiṣe (awọn enzymu diẹ nikan ṣe afihan awọn ipa kataliti to dara julọ).
Ilana katalitiki:
| Awọn enzymu | koodu ọja | koodu ọja |
| Enzymu Powder | ES-AMD-101~ ES-AMD-119 | ṣeto awọn amidases 19, 50 miligiramu kọọkan awọn ohun 19 * 50mg / ohun kan, tabi iye miiran |
| Apo iboju (SynKit) | ES-AMD-1900 | ṣeto ti 19 amidases, 1 miligiramu kọọkan 19 awọn ohun kan * 1mg / ohun kan |
★ Ga sobusitireti pato.
★ Alagbara chiral selectivity.
★ Ga iyipada ṣiṣe.
★ Kere nipasẹ-ọja.
★ Ìwọnba lenu ipo.
★ Ore ayika.
Ṣiṣayẹwo Enzyme yẹ ki o ṣe fun awọn sobusitireti kan pato nitori iyasọtọ sobusitireti, ati gba enzymu kan ti o ṣe itọsi sobusitireti ibi-afẹde pẹlu ipa ayase to dara julọ.
➢ Maṣe kan si awọn ipo to gaju gẹgẹbi: iwọn otutu giga, giga / pH kekere ati ohun elo Organic pẹlu ifọkansi giga.
Ni deede, eto ifaseyin yẹ ki o pẹlu sobusitireti, ojutu ifipamọ (PH ti o dara julọ ti henensiamu).Awọn sobusitireti gẹgẹbi hydroxylamine yẹ ki o wa niwaju ninu eto ifasilẹ gbigbe acyl.
➢ AMD yẹ ki o ṣafikun kẹhin sinu eto ifaseyin pẹlu pH ti o dara julọ ati iwọn otutu.
Gbogbo iru AMD ni ọpọlọpọ awọn ipo ifaseyin to dara julọ, nitorinaa ọkọọkan wọn yẹ ki o ṣe iwadi ni ẹyọkan.
Apeere 1(1):
Iṣẹ ṣiṣe hydrolysis si oriṣiriṣi Amide Sobsitireti
| Sobusitireti | Iṣẹ ṣiṣe pato μmols min-1mg-1 | Sobusitireti | Iṣẹ ṣiṣe pato μmols min-1mg-1 |
| Acetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο- Toluamide | 0.3 |
| Pentanamide | 22.0 | p- Toluamide | 8.1 |
| Hexanamide | 6.4 | Nicotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
Idahun naa ni a ṣe ni 50mM iṣuu soda fosifeti ojutu saarin, pH 7.5, ni 70 ℃.
| Amides | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
Idahun naa ni a ṣe ni 50mM iṣuu soda fosifeti ojutu saarin, pH 7.5, ni 70 ℃.
Ifojusi reagent ti o ni ibatan: amides, 100 mM (benzamide, 10 mM);hydroxylamine ati hydrazine, 400 mM;enzymu 0.9 μM.
Apeere 2(2):
Apeere 3(3):
1. D'Abusco AS, Ammendola S., et al.Extremophiles, 2001, 5:183-192.
2. Guo FM, Wu JP, Yang LR, et al.Biokemisitiri ilana, 2015, 50 (8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, et al.Kemistri Bioorganic, 2017, Wa lori ayelujara 7.
